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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemistry.
Application References:
1. Guleman S, et al. 2009. Mol Cell Biol. 29:1176. PubMed 2. Caton PW, et al. 2010. J. Endocrinol. 205:97. PubMed
HepG2 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-GCN5 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
GCN5 (general control of amino-acid synthesis 5) is a 94 kD member of the N-acetyltransferase superfamily. This nuclear protein is highly expressed in the liver where it functions as a transcriptional co-activator, transfering acetyl group from acetyl-CoA to epsilon amino acid of lysine 14 in core histone H3 to control chromatin remodeling during transcriptional activation. GCN5 is a component of the human STAGA transactivator complex (SPT3, TAF, and GCN5) and can be modified by phosphorylation (which inhibits activity). In addition to the STAGA complex proteins, GCN5 has been shown to interact with E1A and Myc. The Poly6073 antibody has been shown to be useful for Western blotting of the human GCN5 protein. The Poly6073 antibody is directed against the human GCN5 N-terminus.
Other Names:
General control of amino-acid synthesis 5
Structure:
N-acetyltransferase superfamily; approximately 94 kD
Distribution:
Nuclear, highly expressed in liver
Function:
Transcriptional co-activator, transfers acetyl group from acetyl-CoA to epsilon amino acid of lysine 14 in core histone H3 to control chromatin remodeling during transcriptional activation. Component of human STAGA transactivator complex (SPT3, TAF, and G
Regulation:
Phosphorylation inhibits activity
Modification:
Phosphorylation
Interaction:
E1A, Myc
Antigen References:
1. Poux A, et al. 2002. P. Natl. Acad. Sci. USA 99:14065. 2. Candau R, et al. 1996. Mol. Cell. Biol. 16:593. 3. Liu X, et al. 2003. J. Biol. Chem. 278:20405.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-GCN5
HepG2 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-GCN5 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
