Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
293T cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-GCN2 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Description:
This rabbit polyclonal antibody recognizes human GCN2 also known as Eukaryotic translation initiation factor 2 alpha kinase 4, EIF2AK4, GCN2 eIF2alpha kinase and KIAA1338. GCN2 is a ubiquitously expressed cytoplasmic protein, contains a larger region adjacent to the protein kinase domain that is similar in sequence to histidyl-tRNA synthetases(HisRSs), predicted molecular weight approximately 92 kD. GCN2 belongs to a family of stress-responsive protein kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 to regulate general and gene-specific protein synthesis under various cellular stress conditions. GCN2 has been shown to interact with eIF2α protein. The poly6304 antibody has been shown to be useful for Western blotting.
Contains a larger region adjacent to the protein kinase domain that is similar in sequence to histidyl-tRNA synthetases(HisRSs), predicted molecular weight approximately 92 kD
Distribution:
Cytoplasm, ubiquitously expressed
Function:
GCN2 belongs to a family of stress-responsive protein kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 to regulate general and gene-specific protein synthesis under various cellular stress conditions.
Interaction:
eIF2α
Antigen References:
1. Berlanga, J. J., et al. 1999. Europ. J. Biochem. 265:754. 2. Costa-Mattioli, M., et al. 2005. Nature 436:1166.
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