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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.1-0.5 µg antibody per 1 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The optimal concentration should be determined by titration for each individual assay of interest.
Application References:
1 Maestre L, et al. 2009. Haematologica 94:419. 2. Zou L, et al. 2012. J Biol Chem. 287:7190. PubMed.
HeLa cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-GAPDH antibody (clone FF26A/F9). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and chemiluminescence detection.
This mouse monoclonal GAPDH antibody recognizes human GAPDH, also known as glyceraldehyde-3-phosphate dehydrogenase. GAPDH is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. GAPDH is a ubiquitously expressed and has a molecular mass of 36 kD. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. In more recent studies, GAPDH has been shown to be involved in microtubule bundling, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases. The FF26A/F9 monoclonal antibody has been shown to be useful for Western blotting.
Other Names:
glyceraldehyde-3-phosphate dehydrogenase
Structure:
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Distribution:
Ubiquitously expressed, locate in cytoplasm and perinuclear region.
Function:
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and and nicotinamide adenine dinucleotide (NAD). Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretion pathway.
Interaction:
Interacts with TPPP. Interacts with EIF1ADand WARS. Interacts with SUMO4, GLUT4, nPKC-iota and CAMK2
Antigen References:
1. Ercolani L, et al. 1988. J. Biol. Chem. 263:15335. 2. Meyer-Siegler K, et al. 1991. P. Natl. Acad. Sci. USA 88:8460. 3. Hara MR and Snyder SH. 2006. Cell Mol. Neurobiol. 26:527. 4. Zheng L, et al. 2003. Cell 114:255. 5. Wang Q, et al. 2005. FASEB J. 19:869. 6. Bae BI, et al. 2006. P. Natl. Acad. Sci. USA 103:3405.
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Purified anti-GAPDH
HeLa cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-GAPDH antibody (clone FF26A/F9). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and chemiluminescence detection.
