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Purified anti-Galectin-9 Antibody
Purified anti-Galectin-9 Antibody
650701 25 µg ¥21,000     
650702 100 µg ¥45,000     

Product Details

Clone: 9S2-1
Isotype: Mouse IgG1, κ
Reactivity: Human, does not cross react with mouse
Immunogen: Human Galectin-9 (S-type)
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% NaN3.
Preparation: Protein G affinity purified
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted at 4°C.
Application:

WB - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1 µg per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
Enter Lot#:   
Application
References:

1. Pielage JF, et al. 2007. Int. J. Biochem. Cell. Biol. 39:1886. (WB)

Jurkat cell extracts were resolved
Jurkat cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-Galectin-9 antibody (clone 9S2-1). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection.


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Description:

Galectin-9 is a mammalian lectin with a molecular weight around 40 kD. It is a member of the β-galactoside-binding family. With two conserved carbohydrate recognition domains (CRDs), galectin-9 binds small β-galactosides as well as complex glycoconjugates.  HAVCR2/TIM3 has been reported as one of its ligands. Galectin-9 may be retained intracellularly or transported to the cell surface where it can be cleaved to generate a soluble form. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts, and thymus epithelial cells. It can be induced by cytokines in various cell types and is involved in cell aggregation, adhesion, chemotaxis, and apoptosis; galectin-9 induces regulatory T cells and suppresses Th1 and Th17 responses.

Other Names: Lgals9, Gal-9, Ecalectin, Tumor antigen HOM-HD-21, Ecalectin
Structure: 355 amino acids; 40 kD
Distribution: Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells.
Function: Cell aggregation, adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, and induction of regulatory T cells.
Ligand Receptor: HAVCR2/TIM3
Antigen
References:

1. Seki M, et al. 2008. Clin. Immunol. 127:78.
2. Tsubo IY, et al. 2007. Clin. Immunol. 124:221.
3. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
4. Dunphy JL, et al. 2002. J. Biol. Chem. 277:14916.

GeneID: 3965
Latest Publications: View the latest Galectin-9 articles on HighwirePress.com
UniProt: View information about Galectin-9 on UniProt.org
Keywords: Purified anti-Galectin-9, 9S2-1, Purified, Lgals9, Gal-9, Ecalectin, Tumor antigen HOM-HD-21, Ecalectin , Human, does not cross react with mouse, Immunology, Antibodies
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  • Purified anti-Galectin-9
    Jurkat cell extracts were resolved

    Jurkat cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-Galectin-9 antibody (clone 9S2-1). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection.

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