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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1 µg per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References:
1. Pielage JF, et al. 2007. Int. J. Biochem. Cell. Biol. 39:1886. (WB)
Jurkat cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-Galectin-9 antibody (clone 9S2-1). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection.
Galectin-9 is a mammalian lectin with a molecular weight around 40 kD. It is a member of the β-galactoside-binding family. With two conserved carbohydrate recognition domains (CRDs), galectin-9 binds small β-galactosides as well as complex glycoconjugates. HAVCR2/TIM3 has been reported as one of its ligands. Galectin-9 may be retained intracellularly or transported to the cell surface where it can be cleaved to generate a soluble form. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts, and thymus epithelial cells. It can be induced by cytokines in various cell types and is involved in cell aggregation, adhesion, chemotaxis, and apoptosis; galectin-9 induces regulatory T cells and suppresses Th1 and Th17 responses.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Galectin-9
Jurkat cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-Galectin-9 antibody (clone 9S2-1). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection.