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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Berger A, et al. 2010. J. Biol Chem. 285:12248. PubMed 2. Ganglum RK, et al. 2011. J. Biol Chem. 286:3261. PubMed
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Galectin 3 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Galectin 3 (also known as galactose-specific lectin 3 and Mac-2 antigen) is a 32 kD member of the galaptin (S-lectin) family, BH-1, that contains carbohydrate recognition domains. This protein forms homo- and hetero-dimers and localizes to the nucleus or cytoplasm depending on proliferation state. Galectin 3 has been shown to modulate allergic reactions and regulate mRNA splicing activity, cell cycle control, and cell adhesion. The expression of galectin 3 can be increased by acetylated LDL, oxidized LDL, and Runx2. Galectin 3 can be modified by phosphorylation and acetylation and has been shown to interact with IgE, galactose, casein kinase I, laminin, mucin, β-galactoside residues of cell surface, matrix glycoproteins, and some intracellular proteins. The Poly6132 antibody recognizes human and mouse galectin 3 and has been shown to be useful for Western blotting.
IgE, galactose, casein kinase I, laminin, mucin, β-galactoside residues of cell surface, matrix glycoproteins, intracellular proteins
Antigen References:
1. Huflejt M, et al. 1993. J. Biol. Chem. 268:26712. 2. Lotz M, et al. 1993. P. Natl. Acad. Sci. USA 90:3466. 3. Iacobini C, et al. 2003. J. Am. Soc. Nephrol. 14:S264. 4. Takenaka Y, et al. 2003. Cancer Lett. 195:111. 5. Liang G, et al. 2008. Hum Mol Genet. 17:3254.
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Purified anti-Galectin 3
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Galectin 3 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
