This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB1
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Kumar, H., et al., 2005. J. Biol. Chem 280:25928. 2. kumar, P.,et al.2005. J. Biol Chem.280:25928.PubMed
Extracts from 3 day PHA-activated PBMC were resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Fyn antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Description:
Fyn is a 59 kD protein tyrosine kinase and a member of the src subfamily. There are two reported isoforms of Fyn; FynT found in T cells, B cells and thymocytes, and FynB found in neuronal cells. Fyn has been shown to control cell growth, interact with the p85 subunit of PI3K, control high affinity IgE receptor-mediated mast cell degranulation, and participate in neuronal cell signaling. Fyn can be activated by T cell receptor engagement or anti-CD3 ligation. Fyn is known to be modified by phosphorylation and palmitoylation and has been shown to interact with Fyn binding protein, p85 PI3K, high affinity IgE receptor, and NCAM140. The Poly6041 antibody recognizes the N-terminal region of human Fyn and has been shown to be useful for Western blotting.
