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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl antibody per 5 ml antibody dilution buffer for each mini-gel. If primary CD4+CD25+ cells are used, it is recommended that 0.5-1 x 106 cell equivalents be loaded per lane. For immunohistochemical staining of paraffin-embedded sections, the suggested use of this reagent is 1:20 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Sato E, et al. 2005. P. Natl. Acad. Sci. USA 102:18538. (IHC) PubMed 2. Mayack SR and Berg LJ. 2006. J. Immunol. 176:2059. (WB) 3. Gasper-Smith N, et al. 2006. J. Immunol. 177:4670. (WB) 4. Zanin-ZhorovA,et al.2006.J Clin Invest.116:2022. JCI
Western blot analysis of extracts from HEK293T cells transfected with human FoxP3 cDNA, using anti-FoxP3, Poly6238.
The forkhead/winged helix transcription factor FOXP3 is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. The Poly6238 antibody recognizes both human and mouse FOXP3 and has been shown to be useful for Western blotting.
Other Names:
Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Structure:
Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
Distribution:
Nuclear; expressed in T cells
Function:
Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
Regulation:
FOXP3 is present at high levels in T regulatory cell can also be induced by T cell activation
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Purified anti-FOXP3
Western blot analysis of extracts from HEK293T cells transfected with human FoxP3 cDNA, using anti-FoxP3, Poly6238.
