Antibodies
Login
Search   
Purified anti-FOXD3 Antibody
Purified anti-FOXD3 Antibody
631701 50 µl (5 Western blots) £80     
631702 200 µl (20 Western blots) £176     
Product Details
Clone: Poly6317
Isotype: Rabbit IgG
Reactivity: Human
Immunogen: Peptide-KLH
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation: The antibody was purified by antigen-affinity chromatography.
Storage & Handling: Upon receipt, store frozen at -20°C.
Application:

WB, IF
Recommended Usage:

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a starting dilution of 1:100. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Enter Lot#:   
Application
References:

1. Abel EV, et al. 2010. Cancer Res. 70:2891. PubMed
2. Katiyar P, et al. 2011. Mol Cancer Res. 9:545. PubMed.

Whole cell extract from Hela
Whole cell extract from Hela cells (lane 1) and NTERA-2 cells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-FOXD3 antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.


Compare all formats



Description:

The FOXD3 polyclonal antibody recognizes human FOXD3 also known as Forkhead box protein D3 and HNF3/FH transcription factor genesis. FOXD3 belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain, predicted molecular weight 48 kD. FOXD3 binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. FOXD3 is essential for multiple developmental processes in the vertebrate embryo, including neural crest development and maintenance of mammalian stem cell lineages. The poly6317 antibody has been shown to be useful for Western blotting.
Other Names: Forkhead box protein D3, HNF3/FH transcription factor genesis
Structure: FOXD3 belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain, predicted molecular weight 48 kD.
Distribution: Nucleus, expressed in embryonic stem cells
Function: Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. FOXD3 is essential for multiple developmental processes in the vertebrate embryo, including neural crest development
Interaction: Oct4
Antigen
References:

1. Sutton J, et al. 1996. J. Biol. Chem. 271:23126.
2. Hanna LA, et al. 2002. Genes Dev. 16:2650.
3. Guo Y, et al. 2002. Proc. Natl. Acad. Sci. U.S.A. 99:3663.
GeneID: 27022
Latest Publications: View the latest FOXD3 articles on HighwirePress.com
UniProt: View information about FOXD3 on UniProt.org
Keywords: Purified anti-FOXD3, Poly6317, Purified, Forkhead box protein D3, HNF3/FH transcription factor genesis , Human, Immunology, Antibodies
Related Products
DescriptionCloneApplications
HRP Donkey anti-rabbit IgG (minimal x-reactivity)Poly4064ELISA, IHC, WB
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
x
Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-FOXD3
    Whole cell extract from Hela

    Whole cell extract from Hela cells (lane 1) and NTERA-2 cells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-FOXD3 antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.

x
Copyright © 2011 BioLegend, Inc. All rights reserved.