Use at concentrations ≤ 0.5 μg/test. It is recommended that the reagent be titrated for optimal performance for each application.
C57BL/6 thymocytes stained with RM4-5 FITC only (top) or RM4-5 FITC plus purified FIT-22 (bottom), then detected with anti-mouse IgG PE
Description:
The FIT-22 antibody reacts with both fluorescein (FITC) alone and FITC conjugated with antibody or streptavidin. When FIT-22 antibody is used in a three-step staining procedure or separation of FITC-antibody labeled cells, it does not quench FITC fluorescence or react with tested mouse and human cells. The FIT-22 antibody can be used for separation of FITC-antibody labeled cells or as a secondary step to amplify FITC conjugated primary antibody signals (i.e. biotinylated FIT-22 as secondary step, followed by Streptavidin-FITC).
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