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Purified anti-Fluorescein (FITC) Antibody
Purified anti-Fluorescein (FITC) Antibody
408301 50 µg £45     
408302 500 µg £160     
Product Details
Clone: FIT-22
Isotype: Mouse IgG1, κ
Isotype Control:Purified Mouse IgG1, κ Isotype Ctrl
Immunogen: Fluorescein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by Protein-G affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C.
Application:

FC - Quality tested
Recommended Usage:

Use at concentrations ≤ 0.5 μg/test. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Enter Lot#:   
 C57BL/6 thymocytes stained with RM4-5
C57BL/6 thymocytes stained with RM4-5 FITC only (top) or RM4-5 FITC plus purified FIT-22 (bottom), then detected with anti-mouse IgG PE




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Description:

The FIT-22 antibody reacts with both fluorescein (FITC) alone and FITC conjugated with antibody or streptavidin. When FIT-22 antibody is used in a three-step staining procedure or separation of FITC-antibody labeled cells, it does not quench FITC fluorescence or react with tested mouse and human cells. The FIT-22 antibody can be used for separation of FITC-antibody labeled cells or as a secondary step to amplify FITC conjugated primary antibody signals (i.e. biotinylated FIT-22 as secondary step, followed by Streptavidin-FITC).
GeneID: NA
Latest Publications: View the latest FITC articles on HighwirePress.com
UniProt: View information about FITC on UniProt.org
Keywords: Purified anti-Fluorescein (FITC), FIT-22, Purified, Immunology, Antibodies
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Purified Mouse IgG1, κ Isotype CtrlMOPC-21FC, ICC, IF, IHC, IP, WB
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-Fluorescein (FITC)
    C57BL/6 thymocytes stained with RM4-5

    C57BL/6 thymocytes stained with RM4-5 FITC only (top) or RM4-5 FITC plus purified FIT-22 (bottom), then detected with anti-mouse IgG PE





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