This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-FGFR2 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Description:
FGFR2 (fibroblast growth factor receptor 2) is a member of the fibroblast growth factor receptor family containing an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR2 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. FGFR2 is a widely expressed membrane receptor, with distinct isoforms expressed in specific tissues; family members differ in ligand affinities and tissue distribution. This receptor is induced by androgen receptor (prostate) and can be upregulated by TGF-β1 in lung fibroblasts. FGFR2 can be modified by phosphorylation and can bind basic/acidic fibroblast factor or keratinocyte growth factor depending on receptor isoform. ThePoly6163 antibody recognizes human FGFR2 (multiple isoforms) and has been shown to be useful for Western blotting.
Type I membrane protein. Widely expressed membrane receptor, distinct isoforms expressed in specific tissues
Function:
Family members differ in ligand affinities and tissue distribution. Receptor interacts with fibroblast growth factor to induce mitogenesis and differentiation
Regulation:
Induced by androgen receptor expression (prostate), upregulated by TGF-β1 in lung fibroblasts
Modification:
Phosphorylation
Interaction:
Binds basic/acidic fibroblast factor or keratinocyte growth factor depending on isoform
Antigen References:
1. Miki, T., et al., 1992. PNAS 89:246. 2. Dionne, C., et al., 1990. EMBO J. 9:2685. 3. Zhang, Y., et al., 1999. Gene 230:69.
