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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
HepG2 urea lysate was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-Fbx-5 antibody (clone Poly6018). Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
Fbx5 (also known as F-box protein Fbx5, early mitotic inhibitor (Emi1), and F-box only protein 5) is a 44 kD nuclear protein containing an F-box. Fbx5 inhibits APC by binding to Cdc20 and promotes mitotic entry and cyclin A accumulation. Fbx5 is transcriptionally induced by E2F and can be ubiquitinated and targeted for degradation. This protein has been shown to interact with SKP1, Cdc53, Cdc34, Roc1, and Cdc20. The Poly6018 antibody recognizes human Fbx5 and has been shown to be useful for Western blotting.
Other Names:
F-box protein Fbx5, Early mitotic inhibitor (Emi1), F-box only protein 5, FBXO5
Structure:
F-box; 44kD
Distribution:
Nuclear
Function:
Inhibits APC by binding Cdc20. Promotes mitotic entry and cyclin A accumulation.
Regulation:
Transcriptionally induced by E2F
Modification:
Ubiquitination
Interaction:
SKP1, Cdc53, Cdc34, Roc1, Cdc20
Antigen References:
1. Cenciarelli C, et al. 1999. Curr. Biol. 9:1177. 2. Chiaur D, et al. 2000. Cytogenet. Cell Genet. 88:255. 3. Reimann J, et al. 2001. Cell 105:645. 4. Hsu J, et al. 2002. Nat. Cell Biol. 4:358.
Purified anti-Fbx5, Poly6018, Purified, F-box protein Fbx5, Early mitotic inhibitor (Emi1), F-box only protein 5, FBXO5 , Human, Immunology, Antibodies
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Purified anti-Fbx5
HepG2 urea lysate was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-Fbx-5 antibody (clone Poly6018). Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
