Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml of antibody dilution buffer for each mini-gel. For IHC, use a 1:50 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-ERK2 polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
Formalin-fixed paraffin-embedded human testis tissue was stained with Poly6242 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
ERK2 is a 42 kD dual specificity kinase also known as mitogen activated protein kinase 1, extracellular signal regulated kinase 2, MAPK1, and p41mapk. The MAPK family includes several subgroups encompassing the ERK, JNK, and p38 kinases. ERK is phosphorylated in response to various extracellular signals inducing homodimerization and nuclear translocation where it phosphorylates target proteins regulating cell growth and survival. ERK2 interacts with a number of proteins including STAT5A, estrogen receptor alpha, ELK1, MEK1, protein kinase c epilson, RAF1, ribosomal S6 kinase, VAV1, STAT5b, Shc and many others. The Poly6242 antibody recognizes human, mouse, and rat ERK2 and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry.
Other Names:
Mitogen activated protein kinase 1, extracellular signal regulated kinase 2, MAPK1, p41mapk, p42 MAP Kinase
Structure:
Mitogen-activated protein kinase family member, dual specificity kinase, homodimer; approximately 42 kD
Distribution:
Ubiquitous cytoplasmic protein, translocates to nucleus upon activation.
Function:
Dual specificity kinase involved in eukaryotic signal transduction. Phosphorylated ERK2 homodimerizes and translocates from the cytoplasm to the nucelus. Activated ERK2 phosphorylates various targets to regulate cell proliferation, survival and apoptosis.
Regulation:
Extracellular signals including cytokine receptor engagement, growth factor engagement, and neuropeptides induce phosphorylation and activation of ERK2.
Modification:
Auto-phosphorylation on multiple sites (threonine, serine, tyrosine)
Interaction:
Interacts with a variety of proteins including STAT5A, estrogen receptor alpha, ELK1, MEK1, protein kinase c epilson, RAF1, ribosomal S6 kinase, VAV1, STAT5b, Shc and many others.
Antigen References:
1. Khokhlatchev AV, et al. 1998. Cell 93:605. 2. Boulton TG, et al. 1991. Cell 65:663.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-ERK2
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-ERK2 polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
Formalin-fixed paraffin-embedded human testis tissue was stained with Poly6242 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
