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Purified anti-Elongin B Antibody
Purified anti-Elongin B Antibody
629301 25 µg $90.00 Qty:
    
629302 100 µg $195.00 Qty:
    

Product Details

 
Clone: 10C4 (See other available formats)
Isotype: Mouse IgG1, κ
Reactivity: Human
Immunogen: Full length recombinant protein
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB-Quality tested
IP - Reported in the literature
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
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Purified anti-Elongin B Antibody 10C4 Image 1
Hela cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-elongin B (clone 10C4). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.


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Western Blotting Protocol




Description: Elongin B (also known as RNA polymerase II transcription factor SIII p18 subunit and transcription elongation factor B polypeptide 2) is an 18 kD member of the UbH family containing a ubiquitin-like domain. Elongin functions as a subunit of a general transcription elongation factor that increases RNA polymerase II transcription elongation past template-encoded arresting sites in the nucleus. The Elongin BC complex acts as adaptor to link Elongin A, VHL, WSB1 or SOCS1 with a module of CUL2 or CUL5 and RBX1 to form E3 ubiquitin ligases. The 10C4 monoclonal antibody recognizes the human elongin B protein and has been shown to be useful for Western blotting.
Other Names: RNA polymerase II transcription factor SIII p18 subunit, transcription elongation factor B polypeptide 2
Structure: UbH family, ubiquitin-like domain, heterotrimer; 18 kD
Distribution: Nuclear
Function: Regulatory subunit of a general transcription elongation factor that increases RNA polymerase II transcription elongation past template-encoded arresting sites
Interaction: Elongin BC complex acts as adaptor to link Elongin A, VHL, WSB1 or SOCS1 with a module of CUL2 or CUL5 and RBX1 to form E3 ubiquitin ligases
Antigen
References:
1. Duan D, et al. 1995. Science. 269:1402.
2. Stebbins C, et al. 1999. Science. 284:455.
3. Brower C, et al. 2002. P. Natl. Acad. Sci. USA 99:10353.
4. DeRenzo C, et al. 2003. Nature. 424:685.
GeneID: 6923
Latest Publications: View the latest Elongin B articles on HighwirePress.com
UniProt: View information about Elongin B on UniProt.org
Keywords: Purified anti-Elongin B, 10C4, Purified, RNA polymerase II transcription factor SIII p18 subunit, transcription elongation factor B polypeptide 2, Human, Western Blotting, Immunoprecipitation, Immunology, Antibodies


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Related Products

DescriptionCloneApplications
HRP Goat anti-mouse IgG (minimal x-reactivity)Poly4053ELISA, IHC, WB


Other Formats

DescriptionCloneApplications
Biotin anti-Elongin B10C4WB


Related Pathways

Pathway
SOCS Pathway


Related Categories

Category
Cell Biology > DNA Repair/Replication > Elongin B
Cell Biology > Antigens A-Z > Elongin B
Cell Biology > Transcriptional Regulators > Elongin B
Cell Biology > Ubiquitin Conjugation/Degradation > Elongin B

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Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-Elongin B
    Hela cell nuclear extracts were

    Hela cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-elongin B (clone 10C4). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.

  • Biotin anti-Elongin B
    Hela cell nuclear extracts were

    Hela cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-elongin B (clone 10C4). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.

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