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Purified anti-eIF4E Antibody
Purified anti-eIF4E Antibody
606902 200 µl (20 Western blots) ¥45,000     
Product Details
Clone: Poly6069
Isotype: Rabbit IgG
Reactivity: Human
Immunogen: Recombinant (partial), N-terminal
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation: The antibody was purified by antigen-affinity chromatography.
Storage & Handling: Upon receipt, store frozen at -20° C.
Application:

WB - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Enter Lot#:   
 MCF-7 cell extract was resolved
MCF-7 cell extract was resolved by electrophoresis, transferred to nitrocellulose,and probed with rabbitanti-eIF4E antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.


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Description:

eIF4E (also known as eukaryotic translation initiation factor 4E) is a 25 kD member of the eukaryotic initiation factor 4E family. This ubiquitously expressed cytoplasmic protein recognizes and binds 7-methylguanosine containing mRNA "CAP" during initiation of protein synthesis. eIF4E facilitates ribosome binding by inducing unwinding of mRNA secondary structures. Activity of this protein is inhibited by binding of 4E-BP and PRH; expression is increased by H2O2 treatment. Activity can also be regulated by integrin α6β4 ligation. Phosphorylation increases binding to mRNA CAPs and formation of eIF4F complex. eIF4E forms a trimeric complex with eIF4G, eIF4A (called eIF4F). Also associates with 4E-BP and PRH. The Poly6069 antibody has recognizes human eIF4E and has been shown to be useful for Western blotting.
Other Names: Eukaryotic translation initiation factor 4E
Structure: Eukaryotic initiation factor 4E family; 25 kD
Distribution: Cytoplasm, ubiquitously expressed
Function: Recognizes and binds 7-methylguanosine containing mRNA "CAP" during initiation of protein synthesis. Facilitates ribosome binding by inducing unwinding of mRNA secondary structures
Regulation: Inhibited by binding of 4E-BP, PRH. Expression increased by H2O2 treatment. Activity regulated by integrin α6β4 ligation. Phosphorylation increases binding to mRNA CAPs and formation of eIF4F complex
Modification: Phosphorylation
Interaction: Forms trimeric complex with eIF4G, eIF4A (called eIF4F). Also associates with 4E-BP, PRH
Antigen
References:

1. Rychlik W, et al. 1987. P. Natl. Acad. Sci. USA 84:945.
2. Topisirovic I, et al. 2003. EMBO J. 22:689.
3. Shenberger J, et al. 2002. Am. J. Respir. Cell Mol. Biol. 27:250.
GeneID: 13684
Latest Publications: View the latest eIF4E articles on HighwirePress.com
UniProt: View information about eIF4E on UniProt.org
Keywords: Purified anti-eIF4E, Poly6069, Purified, Eukaryotic translation initiation factor 4E, Human, Immunology, Antibodies
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-eIF4E
    MCF-7 cell extract was resolved

    MCF-7 cell extract was resolved by electrophoresis, transferred to nitrocellulose,and probed with rabbitanti-eIF4E antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

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