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Purified anti-eIF3J Antibody
Purified anti-eIF3J Antibody
638401 25 µl $95.00 Qty:
    

Product Details

 
Clone: Poly6384 (See other available formats)
Isotype: Rabbit Polyclonal
Reactivity: Human, Cross-Reactivity: Mouse
Immunogen: Full-length human eIF3J recombinant protein
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation: The antibody was purified by antigen-affinity chromatography.
Storage & Handling: Upon receipt, store frozen at -20°C.
Application:

WB - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
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Purified anti-eIF3J Antibody Poly6384 Image 1
Western blot analysis of extracts from Hela (lane 1) and NIH3T3 cells (lane 2), using anti-eIF3J, Poly6384.


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Western Blotting Protocol




Description:

The eukaryotic translation initiation factor-3 (eIF3) is a 700 kD complex comprised of at least 12 subunits, including eIF3K, which is essential for translation.eIF3 directly binds to the 40S ribosomal subunit and promotes the formation of the 40S preinitiation complex by promoting the binding of methionyl-tRNA and mRNA.Poly6380 is useful for western blot applications.</p

Other Names: Eukaryotic translation initiation factor 3 subunit J, Eukaryotic translation initiation factor 3 subunit 1, eIF3S1, eIF3 p35, eIF3-alpha
Structure: Contains 5 WD repeats. Expected MW is 36 kD.
Distribution: Cytoplasm
Function: eIF3J is component of the eIF3 complex.
Modification: Phosphorylated, Acetylated
Interaction: 40S ribosome subunit, eIF3A, eIF3B, eIF3C, eIF3D, eIF3K
Antigen
References:

1. Block KL, et al. 1998. J. Biol. Chem. 273:31901.
2. Fraser CS, et al. 2007. Mol. Cell. 26:811.

GeneID: 1999
Latest Publications: View the latest eIF3J articles on HighwirePress.com
UniProt: View information about eIF3J on UniProt.org
Keywords: Purified anti-eIF3J, Poly6384, Purified, Eukaryotic translation initiation factor 3 subunit J, Eukaryotic translation initiation factor 3 subunit 1, eIF3S1, eIF3 p35, eIF3-alpha , Human, Cross-Reactivity: Mouse, Western Blotting, Immunology, Antibodies


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Related Categories

Category
Cell Biology > Antigens A-Z > eIF3J
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Version: 1 Revision Date: 2012-11-30
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  • Purified anti-eIF3J
    Western blot analysis of extracts

    Western blot analysis of extracts from Hela (lane 1) and NIH3T3 cells (lane 2), using anti-eIF3J, Poly6384.

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