This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20°C.
Application:
WB
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Western blot analysis of extracts from Hela (lane 1) and NIH3T3 cells (lane 2), using anti-eIF3J, Poly6384.
Description:
The eukaryotic translation initiation factor-3 (eIF3) is a 700 kD complex comprised of at least 13 subunits, including eIF3J, which is essential for translation.eIF3 directly binds to the 40S ribosomal subunit and promotes the formation of the 40S preinitiation complex by promoting the binding of methionyl-tRNA and mRNA. eIF3J is thought to function in part by regulating access of the mRNA-binding cleft in response to initiation factor binding.Poly6384 is useful for western blot applications.
