Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 to 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with purified 11H5 (eIF3/p50) antibody. Proteins were visualized using HRP goat anti-mouse secondary antibody conjugate and a chemiluminescent system.
Description:
The elF3G (eIF3-p40) is a subunit of eukaryotic translation initiation factor 3 (eIF3) complex that plays a central role in the protein translation initiation. The eIF3 consists of at least 10 subunits ranging from 35 to 170 kd. elF3-p40 is constitutively expressed in many tissues, including spleen thymus, prostate, colon, ovary, etc.
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