This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
MCF-7 cell extract was resolved by electrophoresis, transferred to nitrocellulose,and probed with rabbit
anti-eIF-4E antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Description:
eIF-4E (also known as eukaryotic translation initiation factor 4E) is a 25 kD member of the eukaryotic initiation factor 4E family. This ubiquitously expressed cytoplasmic protein recognizes and binds 7-methylguanosine containing mRNA "CAP" during initiation of protein synthesis. eIF-4E facilitates ribosome binding by inducing unwinding of mRNA secondary structures. Activity of this protein is inhibited by binding of 4E-BP and PRH; expression is increased by H2O2 treatment. Activity can also be regulated by integrin α6β4 ligation. Phosphorylation increases binding to mRNA CAPs and formation of eIF4F complex. eIF-4E forms a trimeric complex with eIF-4G, eIF-4A (called eIF-4F). Also associates with 4E-BP and PRH. The Poly6069 antibody has recognizes human eIF-4E and has been shown to be useful for Western blotting.
Other Names:
Eukaryotic translation initiation factor 4E
Structure:
Eukaryotic initiation factor 4E family; 25 kD
Distribution:
Cytoplasm, ubiquitously expressed
Function:
Recognizes and binds 7-methylguanosine containing mRNA "CAP" during initiation of protein synthesis. Facilitates ribosome binding by inducing unwinding of mRNA secondary structures
Regulation:
Inhibited by binding of 4E-BP, PRH. Expression increased by H2O2 treatment. Activity regulated by integrin α6β4 ligation. Phosphorylation increases binding to mRNA CAPs and formation of eIF4F complex
Modification:
Phosphorylation
Interaction:
Forms trimeric complex with eIF-4G, eIF-4A (called eIF-4F). Also associates with 4E-BP, PRH
Antigen References:
1. Rychlik, W., et al., 1987. PNAS 84:945. 2. Topisirovic, I., et al., 2003. EMBO J. 22:689. 3. Shenberger, J., et al., 2002. Am. J. Respir. Cell Mol. Biol. 27:250.
