This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
MCF-7 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-eIF-2γ antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Description:
elF-2γ (also known as eukaryotic translation initiation factor eIF-2 gamma subunit and eukaryotic translation initiation factor 2 subunit 3) is a 52kD member of the GTP-binding elongation factor family, eIF2G subfamily. This cytoplasmic protein is ubiquitously expressed and has GTPase activity involved in the recruitment of methionyl-tRNA to the 40 S ribosomal subunit. Plays a role in translation initiation and elongation. Heterodimeric interaction with eIF-2α and β chains, acts as a cofactor for hepatitis C internal ribosome entry site-mediated translation. The Poly6068 antibody recognizes human eIF-2γ and has been shown to be useful for Western blotting.
