Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
1. Lu, Q., et al. 2010. Am J. Physiol Cell Mol Physiol. 298:501. PubMed.
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit
anti-eIF-2α antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Description:
eIF-2α (also known as eukaryotic translation initiation factor 2, subunit 1 alpha) is a 35 kD member of the eukaryotic translation initiation factor 2 family. This cytoplasmic protein is ubiquitously expressed and can downregulate protein synthesis when phosphorylated by PKR kinase under stress conditions (hypoxia, viral infection, ER stress). Increased expression of eIF-2α has been linked to progression of thyroid carcinoma.Signaling inhibited by p58 (IPK) and PKR phosphorylation. Can attenuate the unfolded protein response and inhibits translation. This protein has been shown to interact with eIF2B α, eIF2B δ, eIF2B β subunit 2, p67, p68, and PKR. eIF-2α is a target for cleavage by caspase 3, caspase 6, caspase 8, and caspase 10. The Poly6067 antibody recognizes both mouse and human eIF-2α and has been shown to be useful for Western blotting.
Downregulates protein synthesis when phosphorylated by PKR kinase under stress conditions (hypoxia, viral infection, ER stress). Increased expressed linked to progression of thyroid carcinoma
Regulation:
Signaling inhibited by p58 (IPK), PKR, attenuates unfolded protein response, inhibits translation
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