Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
1. Lu, Q., et al. 2010. Am J. Physiol Cell Mol Physiol. 298:501. PubMed.
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbitanti-eIF2α antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
eIF2α (also known as eukaryotic translation initiation factor 2, subunit 1 alpha) is a 35 kD member of the eukaryotic translation initiation factor 2 family. This cytoplasmic protein is ubiquitously expressed and can downregulate protein synthesis when phosphorylated by PKR kinase under stress conditions (hypoxia, viral infection, ER stress). Increased expression of eIF2α has been linked to progression of thyroid carcinoma.Signaling inhibited by p58 (IPK) and PKR phosphorylation. Can attenuate the unfolded protein response and inhibits translation. This protein has been shown to interact with eIF2B α, eIF2B δ, eIF2B β subunit 2, p67, p68, and PKR. eIF2α is a target for cleavage by caspase 3, caspase 6, caspase 8, and caspase 10. The Poly6067 antibody recognizes both mouse and human eIF2α and has been shown to be useful for Western blotting.
Downregulates protein synthesis when phosphorylated by PKR kinase under stress conditions (hypoxia, viral infection, ER stress). Increased expressed linked to progression of thyroid carcinoma
Regulation:
Signaling inhibited by p58 (IPK), PKR, attenuates unfolded protein response, inhibits translation
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-eIF2α
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbitanti-eIF2α antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
