This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB,IF
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Chou, H-Y. E., et al., 2006. J. Biol. Chem. 281:15201. 2. Irelan, J. T., et al., 2007. PNAS 104:16940.PubMed
Hela cells were treated with 300 µM mimosine for 16 hrs, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole for an additional 18hrs. Cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit antibody against phosphorylated Eg5 (Thr927). Lane 1, Mimosine only treated Hela cells; Lane 2, mimosine and nocodazole treated Hela. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Description:
hEg5 (also known as kinesin-like protein KIF11, kinesin-related motor protein Eg5, kinesin-like spindle protein HKSP, and thyroid receptor interacting protein 5 (TRIP5)) is a 119 kD kinesin-like protein family, BimC subfamily. This protein is a catalytic kinesin motor with coiled-coil and Smc domains. hEg5 is localized at the centrosomes, spindle microtubules, and intracellular bridge. This motor protein is required for establishing the bipolar spindle. hEg5 is modified by phosphorylation on Thr927 by Cdc2 to allow association with the spindle apparatus. hEg5 has been shown to interact with the thyroid hormone receptor in presence of thyroid hormone and Cdc2. The Poly6205 antibody has been shown to react with phosphorylated human Eg5 (Thr927) by Western blot.
Other Names:
Kinesin-like protein KIF11, kinesin-related motor protein Eg5, kinesin-like spindle protein HKSP, thyroid receptor interacting protein 5 (TRIP5)
Structure:
Kinesin-like protein family, BimC subfamily, kinesin motor catalytic, coiled-coil, Smc domains; 119 kD
