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Purified anti-DYKDDDDK Tag Antibody
Purified anti-DYKDDDDK Tag Antibody
637301 200 µg $215.00     
637302 500 µg $325.00     
637303 1 mg $405.00     
637304 5 mg $665.00     
Product Details
Clone: L5
Isotype: Rat IgG2a, κ
Reactivity: DYKDDDDK tag epitope
Immunogen: DYKDDDDK-tagged mouse Langerin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C.
Application:

WB - Quality tested
IP, IF, IHC, ELISA, FC - Reported in the literature
Recommended Usage:

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Enter Lot#:   
Application
References:

1. Park SH, et al. 2008. J Immunol Methods. 331:27.
2. Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
3. Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed.

Cell extracts from untransfected 293T
Cell extracts from untransfected 293T cells (lane 1) or 293T cells transfected with a plasmid encoding DYKDDDDK-tagged protein (lane 2), using anti-DYKDDDDK, clone L5.


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Description:

The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.
Other Names: FLAG tag
Antigen
References:

1. Einhauer A. 2001. J. Biochem. Biophys. Methods. 49:455.
2. Knappik A and Pluckthun A. 1994. Biotechniques. 17:754.
GeneID: NA
Latest Publications: View the latest DYKDDDDK articles on HighwirePress.com
UniProt: View information about DYKDDDDK on UniProt.org
Keywords: Purified anti-DYKDDDDK Tag, L5, Purified, FLAG tag , DYKDDDDK tag epitope, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-DYKDDDDK Tag
    Cell extracts from untransfected 293T

    Cell extracts from untransfected 293T cells (lane 1) or 293T cells transfected with a plasmid encoding DYKDDDDK-tagged protein (lane 2), using anti-DYKDDDDK, clone L5.

  • Anti-DYKDDDDK Tag (L5) Affinity Gel
    Anti-DYKDDDDK tag affinity resin (L5-agarose)

    Anti-DYKDDDDK tag affinity resin (L5-agarose) retains binding capacity even after 10 rounds of purification and elution.

    SDS PAGE gel image of

    SDS PAGE gel image of protein purified from the 1st and 10th rounds by either L5 agarose or competitor's equivalent product.

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