This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol. Final antibody concentration is 0.5 mg/ml.
Preparation:
The antibody was purified by protein G affinity chromatography
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 μg per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1~4 μg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
MCF-7 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse anti-DNA-PKcs (clone 7A4). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Description:
DNA-protein kinase catalytic subunit (DNA-PKcs) also known as DNA-activated kinase is a nuclear 460-470 kD serine threonine kinase involved in double-stranded DNA break repair, VDJ recombination, and transcriptional modulation. DNA-PKcs must bind DNA ends to become active. DNA-PKcs is modified by phosphorylation and has been shown to interact with Ku70/Ku80, KIP, DNA-ligase IV, and XRCC4 proteins. The 7A4 monoclonal antibody has recognizes human DNA-PKcs and has been shown to be useful for immunofluorescence staining and Western blotting.
Other Names:
DNA-protein kinase catalytic subunit, DNA-activated protein kinase, DNA-dependent protein kinase
1. Hartley, K., et al., 1995. Cell. 82:849. 2. Connelly, M., et al., 1996. Gene. 175:271. 3. Douglas, P., et al., 2002. Biochem. J. 368:243 4. Calsou, P., et al., 2003. J. Mol. Biol. 326:93.
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