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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 μg antibody per 5 ml antibody dilution buffer for each minigel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
HeLa cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Daxx antibody (clone DAXX-1). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection.
The DAXX-01 monoclonal antibody recognizes human Daxx also known as Death domain-associated protein 6 and EAP1. Daxx is a ubiquitously expressed protein with a predicted molecular weight approximately 81 kD. Daxx binds to both Fas death domain and the apoptosis signal-regulating kinase 1 (ASK1) and promotes Fas-mediated apoptosis through a signal transduction pathway involving the Jun amino-terminal kinase(JNK) pathway. Daxx is mainly located in the nucleus as a transcriptional repressor and also located in the cytoplasmic as a proapoptotic signal mediator. The DAXX-01 antibody has been shown to be useful for Western blotting and Immunocytochemistry. Daxx migrated with an apparent molecular weight of approximately 120 kD on SDS-PAGE; this slower than expected migration may reflect the high content of acidic residues in Daxx.
Other Names:
Death domain-associated protein 6, EAP1
Structure:
Daxx is an adaptor protein, has neither a death nor a TRAF domain, predicted molecular weight approximately 81 kD
Distribution:
Ubiquitously expressed
Function:
Daxx binds to both Fas death domain and the apoptosis signal-regulating kinase 1 (ASK1) and promotes Fas-mediated apoptosis through a signal transduction pathway involving the Jun amino-terminal kinase(JNK) pathway. Daxx is mainly located in the nucleus a
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Purified anti-Daxx
HeLa cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Daxx antibody (clone DAXX-1). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection.
