Reacts with conserved epitope in all species tested. Recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18
Immunogen:
Keratin enriched fraction from human epidermoid carcinoma
Formulation:
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation:
The antibody was purified by affinity chromatography.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4 °C.
Application:
WB - Quality Tested IP*, IHC(Paraffin)*, ICC*, IF * - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 μg antibody per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1-4 μg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Kovarik, J., 1988. Int. J. Cancer Suppl. 3:50. 2. Bartek, J., et al., 1991. J. Pathol. 164:215. 3. Chernyavsky, A. I., 2007. J. Biol. Chem. doi:10.1074/jbc.M611365200.PubMed
Hela cells stained with purified mouse monoclonal antibody against pan Cytokeratin (clone C-11), followed by Rhodamine Red-X conjugated Donkey anti-mouse IgG and DAPI
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal ant-cytokeratin antibody (clone C-11). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Description:
Cytokeratins are intermediate filament proteins that are widely expressed in many tissues. These proteins are vital components of the cytoskeleton and interact with a number of other proteins. Cytokeratins are usually found in the cytoplasm,although some cytokeratins can be found in the nucleus and the nucleolus. The C-11 monoclonal antibody reacts with conserved epitope in all species tested of cytokeratin 4, 5, 6, 8, 10, 13, and 18. This antibody has been shown to be useful for Western blotting and has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), and immunocytochemistry.
Other Names:
Cytokeratin, Keratin
Structure:
Intermediate filament protein, contains three coiled-coil domains. Cytokeratins exist as heterotetramers composed of two type I and two type II keratin subunits
Distribution:
Cytokeratins are widely expressed proteins usually found in the cytoplasm although some cytokeratins can also be found in the nucleus and the nucleolus
Function:
Intermediate filament protein involved with the cytoskeleton.
Interaction:
Most cytokeratins interact with a number of other proteins such as 14-3-3, EGF receptor, and many others
