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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Vojtesek B, et al. 1990. Neoplasma 37:333.
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-cytokeratin 7 + 17 antibody (clone C-46). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Cytokeratins are proteins that are involved in the cytoskeleton. The cytokeratin proteins are heterotetramers composed of two type I and two type II subunits. Cytokeratin 17 is a type I protein and cytokeratin 7 is a type II intermediate filament protein, each protein contains three coiled-coil domains. Cytokeratin 7 has an approximate molecular weight of 54 kD; cytokeratin 17 has an approximate molecular weight of 46 kD. Cytokeratin 17 is expressed in the basal cells of complex epithelia, but not in stratified or simple epithelia. Cytokeratin 7 is expressed in the epidermis, bronchus, and mesothelium but not in the colon, exocervix or liver. Mutations in cytokeratin 17 have been shown to result in pachyonychia congenital. Cytokeratin 17 has been shown to interact with the EGF receptor. Cytokeratin 7 has been shown to interact with the EGF receptor and heat shock protein 70 kD1A. The C-46 monoclonal antibody recognizes human, bovine and porcine (but not mouse, rat, or rabbit) cytokeratin 7 and cytokeratin 17 and is useful for Western blotting.This antibody has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), and immunocytochemistry.
Other Names:
Keratin 7/keratin17, Keratin 7/17
Structure:
Type I (cytokeratin 17) and type II (cytokeratin 7) intermediate filament protein, contains three coiled-coil domains. Cytokeratins are heterotetramers composed of two type I and two type II keratins. Cytokeratin 7 has an approximate molecular weight of 5
Distribution:
Cytokeratin 17 is expressed in the basal cells of complex epithelia, but not in stratified or simple epithelia. Cytokeratin 7 is expressed in the epidermis, bronchus, and mesothelium but not in the colon, exocervix or liver.
Function:
Intermediate filament protein involved with the cytoskeleton. Mutations in cytokeratin 17 have been shown to result in pachyonychia congenita
Interaction:
Cytokeratin 17 has been shown to interact with the EGF receptor. Cytokeratin 7 has been shown to interact with the EGF receptor and heat shock protein 70 kD1A.
Antigen References:
1. Glass C, et al. 1988. J. Cell Biol. 107:1337. 2. Troyanovsky SM, et al. 1992. Eur. J. Cell Biol. 59:127. 3. Terrinoni A, et al. 2001. J. Invest. Dermatol. 117:1391.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Cytokeratin 7/17
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-cytokeratin 7 + 17 antibody (clone C-46). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
