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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. KarstenU, et al. 1985. Eur. J. Cancer Clin. Oncol. 21:733.
MCF-7 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-cytokeratin 19 antibody (clone A53-B/A2). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
MCF-7 cells were stained with anti-Cytokeratin 19 (clone A53-B/A2), followed by Alexa Fluor® 546 secondary antibody and DAPI (nuclei). Images were aquired on a Nikon FC300 inverted microscope at 20X magnification. Data provided by Dr. John Nolan, La Jolla Bioengineering Institute.
Cytokeratin 19, also known as keratin 19, is a type I intermediate filament protein with a molecular weight of approximately 40-44 kD. Cytokeratin 19 is a heterotetramer composed of two type I and two type II keratin subunits. Unlike other cytokeratins, cytokeratin 19 lacks a C-terminal non-helical extension. This cytokeratin is widely expressed in the periderm (transient superficial layer enveloping developing epidermis), muscle, intestine, bile duct, esophagus, stomach, and thymus. Cytokeratin 19 can be upregulated by vitamin A and is thought to play a critical role in embryogenesis. Cytokeratin 19 intereacts with the pinin protein and has been shown to be modified by phosphorylation (Ser10, Ser35).The A53-B/A2 monoclonal antibody recognizes human cytokeratin 19 and is useful for Western blotting. This antibody has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), immunocytochemistry, and ELISA.
Other Names:
Keratin 19, Keratin 19 type 1 cytoskeletal
Structure:
Type I intermediate filament protein, contains three coiled-coil domains, lacks C-terminal non-helical extension found in other keratins, approximately 40-44 kD. Cytokeratin 19 is a heterotetramer composed of two type I and two type II keratin subunits.
Distribution:
Expressed in the periderm (transient superficial layer enveloping developing epidermis), also expressed in muscle, intestine, bile duct, esophagus, stomach, and thymus. Upregulated by vitamin A.
Function:
Intermediate filament protein involved with the cytoskeleton. May play a critical role in embryogenesis
Modification:
Phosphorylation (Ser10, Ser35)
Interaction:
Interacts with pinin
Antigen References:
1. Bader BL, et al. 1986. EMBO J. 5:1865. 2. Eckert RL. 1988. Proc. Natl. Acad. Sci. 85:1114. 3. Stasiak PC and Lane EB. 1987 Nucleic Acids Res. 15:10058.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Cytokeratin 19
MCF-7 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-cytokeratin 19 antibody (clone A53-B/A2). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
MCF-7 cells were stained with anti-Cytokeratin 19 (clone A53-B/A2), followed by Alexa Fluor® 546 secondary antibody and DAPI (nuclei). Images were aquired on a Nikon FC300 inverted microscope at 20X magnification. Data provided by Dr. John Nolan, La Jolla Bioengineering Institute.
