This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-cytochrome p450-2B6 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Description:
Cytochrome p450-2B6 is a member of the cytochrome P450 superfamily. Multiple splice variants of this protein exist, the approximate molecular weight of cytochrome p450-2B6 is 56 kD. This protein is membrane-bound and can be found in the endoplasmic reticulum. Ubiquitously expressed, cytochrome p450-2B6 is moderately to highly expressed in liver microsomes. This protein functions as a heme-thiolate monooxygenase involved in NADPH-dependent electron transport pathway oxidizing a variety of compounds including steroids, fatty acids, and xenobiotics. Cytochrome p450-2B6 is induced by phenobarbital, and active vitamin D. Phosphorylation has been shown to decrease enzyme activity. Cytochrome p450-2B6 binds to a variety of steroids, fatty acids and xenobiotics. The Poly6178 antibody has been shown to be useful for the Western blotting of human cytochrome p450-2B6.
Other Names:
CYP2B6
Structure:
Cytochrome P450 superfamily. Splice variants, approximately 56 kD
Distribution:
Membrane-bound, endoplasmic reticulum. Ubiquitously expressed, moderate to high expression in liver microsomes
Function:
Heme-thiolate monooxygenase involved in NADPH-dependent electron transport pathway. Oxidizes a variety of compounds including steroids, fatty acids, and xenobiotics
Regulation:
Induced by phenobarbital, active vitamin D. Phosphorylation decreases enzyme activity
Modification:
Phosphorylation
Interaction:
Binds to a variety of steroids, fatty acids and xenobiotics
Antigen References:
1. Ekins, S., et al., 1999. Drug Metab. Rev. 31:719. 2. Gervot, L., et al., 1999. Pharmcogenetics 9:295.
