This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation:
The antibody was purified by protein G affinity chromatography
Storage & Handling:
Upon receipt, store undiluted at 4° C.
Application:
WB
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Jemmerson, R., et al., 1991. Eur. J. Immunol. 21:143.
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-cytochrome C (clone 7H8.2C12) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Description:
Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 7H8.2C12 monoclonal antibody recognizes cytochrome-c from most species and has been shown to be useful for Western blotting.
Other Names:
Cyt c
Structure:
Heme binding domain; 15 kD
Distribution:
Mitochondrial intermembrane space
Function:
Component of electron transport chain; heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9
