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Purified Anti-Cytochrome C Antibody
Material Safety Data Sheet (MSDS) Purified Anti-Cytochrome C Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Purified Anti-Cytochrome C Antibody
612301 25 µg $70.00     
612302 100 µg $170.00     
Clone: 6H2.B4
Isotype: Mouse IgG1, κ
Reactivity: Mouse, Rat, Human
Immunogen: Rat cyt c-OVA
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation: The antibody was purified by protein G affinity chromatography
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store at 4° C.
Application: ICFC5-Quality Tested
IP4, IF3,5, ICC5* - *This application has been reported in the literature.
Recommended Usage: This antibody can be used at 2-4 μg /1 x107 cell equivalents for immunoprecipitation. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume.
It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
Additional reported applications (for the relevant formats) include: intracellular flow cytometry5, immunofluorescence microscopy3,5, immunoprecipitation4, Western blotting4,5, and immunocytochemistry5.
Application
References:
1. Goshorn, SC. et al., 1991. J. Biol. Chem. 266:2134.
2. Jemmerson, R., et al., 1991. Eur. J. Immunol. 21:143.
3. Chandra, D., et al. 2002. J. Biol. Chem. 277:50842.
4. Semenkova, L., et al., 2003. Eur. J. Biochem. 270:4388.
5. Shih, S-F., et al., 2001. J. Biol. Chem. 276:21870.
Cytochrome c was immunoprecipitated from

Cytochrome c was immunoprecipitated from Hela cell extract (1% NP-40) using 2-4 µg 6H2.B4 antibody/1 x107 cell equivalents. Immunoprecipitates were resolved by electrophoresis, transferred to nitrocellulose, and probed with the 7H8.2C12 anti-cytochrome c antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. In addition to the specific 15 kD cytochrome c band immunoprecipitated by 6H2.B4, heavy and light immunoglobulin chains are recognized by the goat anti-mouse secondary antibody.


Description: Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 6H2.B4 monoclonal antibody recognizes human, mouse, and rat cytochrome-c and has been shown to be useful for intracellular flow cytometric staining, Western blotting, immunoprecipitation, and immunofluorescence staining.
Other Names: Cyt c
Structure: Heme binding domain; 15 kD
Distribution: Mitochondrial intermembrane space
Function: Component of electron transport chain; heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9
Interaction: Cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, Casp9
Antigen
References:
1. Liu, X., et al., 1996. Cell. 86:147.
2. Li, P., et al., 1997. Cell. 91:479.
3. Zhang, Z., et al., 2003. Gene 312:61.
4. Ferguson, H., et al., 2003. J. Biol. Chem. 278:45793.
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HRP Goat anti-mouse IgG (minimal x-reactivity)Poly4053ELISA,IHC,WB
Purified anti-Cytochrome c7H8.2C12WB
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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