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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 1:100~400 ml antibody dilution buffer for each mini-gel. For IHC, use a 1:100 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Song SY, et al. 2005. Int J Mol Med. 16:517.
NIH 3T3 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Cyclin E antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system
Formalin-fixed paraffin-embedded human placenta tissue was stained with Poly6307 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
This rabbit polyclonal antibody recognizes human, mouse, and rat cyclin E also known as Cyclin E1, CCNE, G1/S specific cyclin E1. Cycline E is a nucleus protein, predicted molecular weight 47 kD. Cycline E belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cycline E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E is essential for the control of the cell cycle at the G1/S transition. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. The poly6307 antibody has been shown to be useful for Western blotting, Immunoprecipitation, and Immunohistochemistry.
Other Names:
Cyclin E1, CCNE, G1/S specific cyclin E1
Structure:
Belongs to the highly conserved cyclin family, predicted molecular weight 47 kD.
Distribution:
Nucleus protein, ubiquitous expressed
Function:
Essential for the control of the cell cycle at the G1/S (start) transition.
Interaction:
Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with retinoblastoma binding protein 3 and retinoblastoma-like protein
Antigen References:
1. Koff A, et al. 1991. Cell 66:1217. 2. Mumberg D, et al. 1997.Nucl. Acids Res. 25:2098. 3. Prall OW, et al. 1997. J. Biol. Chem. 272(16):10882.
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Purified anti-Cyclin E
NIH 3T3 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Cyclin E antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system
Formalin-fixed paraffin-embedded human placenta tissue was stained with Poly6307 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
