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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 2.5-5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications include: immunohistochemistry2 of formalin-fixed paraffin-embedded sections and immunofluorescence1 of methanol fixed cells grown on glass coverslips.
Application References:
1. Geley S, et al. 2001. J. Cell Biol. 153:137-148 PubMed. 2. Brandeis M, et al. 1998. P. Natl. Acad. Sci. USA 95:4344-4349. PubMed 3. Matsumura F, et al. 2011. J. Biol Chem. PubMed
Western blot analysis of extracts from Hela (lane 1) and NIH/3T3 (lane 2) cells, using anti-Cyclin B1, V152.
Cyclin B1 belongs to the cyclin family, Cyclin AB subfamily, and predicted molecular weight 48 kD. Cyclin B1 is expressed ubiquitously in all eukaryotes and the level of cyclin B1 was minimal in G, began to rise in S phase, and is primarily cytoplasmic. Cyclin B1 moves into the nucleus just at the start of mitosis and associates with condensed chromosomes and the mitotic spindle. The level of cyclin B1 peaked at the G/M transition. Cyclin B1 is essential for the control of the cell cycle at the G2/M transition. The V152 monoclonal antibody recognizes human and mouse Cyclin B1 and has been shown to be useful for Western blotting. and immunohistochemistry (paraffin embedded tissue sections) of human and mouse cyclin B1.
Other Names:
CCNB1
Structure:
Belongs to the cyclin family. Cyclin AB subfamily.
Distribution:
Ubiquitous expressed in all eukaryotes, predicted molecular weight 48 kD.
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Purified anti-Cyclin B1
Western blot analysis of extracts from Hela (lane 1) and NIH/3T3 (lane 2) cells, using anti-Cyclin B1, V152.
