Peptide mapping to the carboxy terminus of human cyclin B1
Formulation:
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.
Preparation:
This antibody was purified by affinity chromatography.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. DO NOT FREEZE.
Application:
WB-Quality Tested
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Western blot analysis of extracts from Hela cells, using anti-Cyclin B1, Poly6334.
Description:
Cyclin B1 belongs to the cyclin family, Cyclin AB subfamily, and predicted molecular weight 48 kD. Cyclin B1 is expressed ubiquitously in all eukaryotes and the level of cyclin B1 was minimal in G, began to rise in S phase, and is primarily cytoplasmic. Cyclin B1 moves into the nucleus just at the start of mitosis and associates with condensed chromosomes and the mitotic spindle. The level of cyclin B1 peaked at the G/M transition. Cyclin B1 is essential for the control of the cell cycle at the G2/M transition. The poly6334 antibody has been shown to be useful for western blotting and immunohistochemistry of human, mouse, and rat cyclin B1.
Other Names:
CCNB1
Structure:
Belongs to the cyclin family. Cyclin AB subfamily.
Distribution:
Ubiquitous expressed in all eukaryotes, predicted molecular weight 48 kD.
