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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5-1 µg per ml antibody dilution buffer. It is recommended that the reagent be titrated for optimal performance for each application.
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HeLa cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with polyclonal anti-cyclin A antibody (clone E23.1). Proteins were visualized using a goat anti-mouse IgG conjugated to HRP and chemiluminescence detection.
Cyclins regulate the activity of the Cyclin-dependent protein kinases. Cyclin A is involved in the regulation of the cell cycle and seems to be essiential for S phase and M phase. Studies have shown that overexpression of cyclin A can accelerate the G1 to S phase. Cyclin A is a marker for actively proliferating cells and for cells in S phase.
Distribution:
55 kd protein
Antigen References:
1. Pagano M et al. 1992. EMBO J 11:961 2. Geley S et al. 2001 J Cell Biol. 153:137 3. Resinitzky D et al. 1995. Mol Cell Biol 15:4347 4. Furuno N et al. 1999. J Cell Biol. 147:295
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Cyclin A
HeLa cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with polyclonal anti-cyclin A antibody (clone E23.1). Proteins were visualized using a goat anti-mouse IgG conjugated to HRP and chemiluminescence detection.