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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-CPF antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
CPF, also known as nuclear receptor subfamily 5 group A member 2, orphan nuclear receptor NR5A2, HB1F, fetoprotein transcription factor, FTF, CYP7A promoter binding factor, and LRH1 is a 54 kD nuclear receptor protein. CPF is a member of the FTZ-F1 sub-family and is highly expressed in the pancreas and to a lesser extent in the liver. CPF contains a DNA binding domain, a hormone ligand-binding domain, and two zinc finger domains and binds to DNA as a monomer. CPF is known to regulate transcription of the liver enzyme cholesterol 7-alpha-hydrolase that converts cholesterol to bile acids and to activate the hepatitis B virus enhancer II to contribute to the liver-specific expression of this virus. CPF interacts with multiprotein bridging actor 1 and the orphan nuclear receptor SHP and has been suggested to regulate pancreas-specific gene expression and play a role in embryonic development. There are at least 3 alternatively spliced isoforms of CPF; isoforms 1 and 2 are present in fetal and adult liver and in liver cancer cells such as HepG2. The Poly6066 recognizes human CPF (amino acids 1-67) and has been shown to be useful for Western blotting.
Other Names:
Nuclear receptor subfamily 5 group A member 2, orphan nuclear receptor NR5A2, HB1F, fetoprotein transcription factor, FTF, CYP7A promoter binding factor, LRH1
Structure:
Orphan nuclear receptor, member of the FTZ-F1 sub-family, contains a DNA binding domain, 2 zinc finger motifs, an FTZ-F1 box and a ligand-binding domain; approximately 54 kD, three alternatively spliced variants exist
Distribution:
Nuclear; highly expressed in pancreas, lower expression in liver, very low expression in heart and lung
Function:
Binds DNA as a monomer to regulate transcription of the liver enzyme cholesterol 7-alpha-hydroxylase converting cholesterol to bile acids; activates viral hepatitis B enhancer II to regulate liver-specific expression of this virus in combination with othe
Regulation:
Expression is activated by HNF1 and HNF3. Interaction with SHP NRB02 inhibits CPF-mediated transactivation
1. Li M, et al. 1998. J. Biol. Chem. 273:29022. 2. Nitta M, et al. 1999. P. Natl. Acad. Sci. USA 96:6660. 3. Lee YK, et al. 2002. J. Biol. Chem. 277:2463. 4. Zhang CK, et al. 2001. Gene 273:239.
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Purified anti-CPF
Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-CPF antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
