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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Antibody was tested using uninduced E. coli cell lysates (lane 1) or E. coli cell lysates induced with IPTG (lane 2) to express CHOP. Total lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anto-CHOP antibody (Poly6436) antibody. The proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection.
CHOP (also known as growth arrest and DNA-damage-inducible protein GADD153) is a 27 kD member of the C/EBP transcription factor family containing a basic region involved in DNA binding and a leucine zipper domain. This nuclear protein functions as a dimer to inhibit the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA target sequences. CHOP (GADD153) is induced by endoplasmic-reticulum stress and nutrient deprivation. Phosphorylation by p38-MAP kinase enhances activity of this protein while CK2 phosphorylation decreases activity. CHOP (GADD153) has been shown to interact with c-jun, c-fos, C/EBP, and LAP. The Poly6436 antibody has been shown to be useful for Western blotting of the human CHOP protein.
Other Names:
Growth arrest and DNA-damage-inducible protein GADD153, DNA-damage inducible transcript 3, C/EBP-homologous protein
Structure:
C/EBP transcription factor family, basic region involved in DNA binding, leucine zipper domain, dimer; 27 kD
Distribution:
Nuclear
Function:
Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA target sequences.
Regulation:
Induced by endoplasmic-reticulum stress and nutrient deprivation. Phosphorylation by p38-MAP kinase enhances activity. CK2 phosphorylation decreases activity.
Modification:
Phosphorylation
Interaction:
c-jun, c-fos, C/EBP, LAP
Antigen References:
1. Ron D, et al. 1992. Genes Dev. 6:439. 2. Ubeda M, et al. 1999. Mol. Cell. Biol. 19:7589. 3. Ubeda M, et al. 2003. J. Biol. Chem. 278:40514.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-CHOP (GADD153)
Antibody was tested using uninduced E. coli cell lysates (lane 1) or E. coli cell lysates induced with IPTG (lane 2) to express CHOP. Total lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anto-CHOP antibody (Poly6436) antibody. The proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection.
