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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
293 T cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with mouse anti-Chk2 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Chk2 (also known as cell cycle checkpoint kinase 2) is a serine/threonine protein kinase containing an FHA domain. There are two reported isoforms of Chk2 (designated A and B) with molecular weights of approximately 60 kD. Chk2 can be modified by phosphorylation. This nuclear protein controls cell cycle checkpoint and inhibits Cdc25C by phosphorylation. Chk2 prevents entry into mitosis and is upregulated by autophosphorylation in response to DNA damage or other agents that cause replication block. Chk2 has been shown to interact with BRCA1, p53, and Cdc25C. The CHK2909 monoclonal antibody has been shown to be useful for Western blotting of the human Chk2 protein.
Other Names:
hCds1, CHEK2, RAD53, Checkpoint kinase 2
Structure:
Serine/Threonine protein kinase, FHA domain.Two isoforms (A and B) both approximately 60 kD
Distribution:
Nuclear
Function:
Controls cell cycle checkpoint, inhibits Cdc25C by phosphorylation. Prevents entry into mitosis
Regulation:
Upregulated by autophosphorylation in response to DNA damage, replication block
Modification:
Phosphorylation
Interaction:
BRCA1, p53, Cdc25C
Antigen References:
1. Wu X, et al. 2001. J. Biol. Chem. 276:2971. 2. Iliakis G, et al. 2003. Oncogene. 22:5834. 3. Foray N, et al. 2003. EMBO J. 22:2860. 4. Matsuoka S, et al. 1998. Science. 282:1893.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Chk2
293 T cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with mouse anti-Chk2 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
