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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
This antibody is not suitable for immunoprecipitation.
Application References:
1. Appierto V, et al. 2009. Mol Cancer Res. 8:3360. PubMed
HepG2 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal antibody against ch-TOG. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
ch-TOG (also known as colonic and hepatic tumor over-expressed protein) is a 218 kD member of the TOG/XMAP215 family containing heat repeats. This protein is localized in the perinuclear cytoplasm during interphase and at the spindle poles and centrosomes during mitosis. Ch-TOG is believed to function in spindle microtubule organization and has been shown to interact with TACC1, TACC3,and tubulin. The Poly6204 antibody has be shown to be useful for Western blotting of the human ch-TOG protein.
Other Names:
Colonic and hepatic tumor over-expressed protein, KIAA0097
1. Charrasse S, et al. 1995. Eur. J. Biochem. 234:406. 2. Garcia M, et al. 2001. EMBO J. 20:3389. 3. Lauffart B, et al. 2002. Biochem. J. 363:195. 4. Gergely F, et al. 2003. Genes Dev. 17:336.
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Purified anti-ch-TOG
HepG2 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal antibody against ch-TOG. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
