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Purified Anti-Centrin 2 (Caltractin) Antibody
Material Safety Data Sheet (MSDS) Purified Anti-Centrin 2 Caltractin Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Purified Anti-Centrin 2 (Caltractin) Antibody
628801 50 µl (5 Western blots) $100.00     
628802 200 µl (20 Western blots) $250.00     
Clone: poly6288
Isotype: Rabbit IgG
Reactivity: Human
Immunogen: Recombinant centrin 2 protein
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation: The antibody was purified by antigen-affinity chromatography.
Storage & Handling: Upon receipt, store frozen at -20° C.
Application: WB
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Hela cell extract was resolved

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-centrin 2 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.


Description: Centrin 2, also known as caltractin, EF-hand protein 2, and 20 kD calcium-binding protein, is a calcium-binding protein that contains 4 EF-hand domains. Centrin 2 is highly expressed in the retina and testis, also expressed in liver, skeletal muscle and cardiac muscle. The centrin-2 protein plays a critical function in normal cell division,and is known to associate with the centrosomes, mitotic spindle poles, and basal bodies. Centrin 2 has a fundamental role in the structure and function of the microtubule-organizing center and has been shown to associate with the RAD23B, SFI1, and Xeroderma pigmentosum group C complementing proteins. The Poly6288 antibody recognizes human centrin 2 and has been shown to be useful for Western blotting.
Other Names: EF-hand protein 2, 20 kD calcium-binding protein, Caltractin
Structure: Contains 4 EF-hand domains, calcium binding protein, approximately 20-21 kD
Distribution: Highly expressed in the retina and testis, also expressed in liver, skeletal muscle and cardiac muscle
Function: Plays a critical function in normal cell division, associates with the centrosomes, mitotic spindle poles, and basal bodies. Has a fundamental role in the structure and function of the microtubule-organizing center.
Interaction: RAD23B, SFI1, Xeroderma pigmentosum group C complementing protein
Antigen
References:
1. Lee, V. D., and Huang, B. 1993. Proc. Natl. Acad. Sci. 90:11039.
2. Tanaka, T., et al., 1994. Genomics 24:609.
HRP Donkey anti-rabbit IgG (minimal x-reactivity)Poly4064ELISA,IHC,WB