This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-centrin 2 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Description:
Centrin 2, also known as caltractin, EF-hand protein 2, and 20 kD calcium-binding protein, is a calcium-binding protein that contains 4 EF-hand domains. Centrin 2 is highly expressed in the retina and testis, also expressed in liver, skeletal muscle and cardiac muscle. The centrin-2 protein plays a critical function in normal cell division,and is known to associate with the centrosomes, mitotic spindle poles, and basal bodies. Centrin 2 has a fundamental role in the structure and function of the microtubule-organizing center and has been shown to associate with the RAD23B, SFI1, and Xeroderma pigmentosum group C complementing proteins. The Poly6288 antibody recognizes human centrin 2 and has been shown to be useful for Western blotting.
Other Names:
EF-hand protein 2, 20 kD calcium-binding protein, Caltractin
Structure:
Contains 4 EF-hand domains, calcium binding protein, approximately 20-21 kD
Distribution:
Highly expressed in the retina and testis, also expressed in liver, skeletal muscle and cardiac muscle
Function:
Plays a critical function in normal cell division, associates with the centrosomes, mitotic spindle poles, and basal bodies. Has a fundamental role in the structure and function of the microtubule-organizing center.
Interaction:
RAD23B, SFI1, Xeroderma pigmentosum group C complementing protein
Antigen References:
1. Lee, V. D., and Huang, B. 1993. Proc. Natl. Acad. Sci. 90:11039. 2. Tanaka, T., et al., 1994. Genomics 24:609.
