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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Hela cells were treated with 300 µM mimosine for 16 hrs, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole (lane 2) for an additional 18hrs. Nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit polyclonal antibody reactive against phosphorylated Cdc25A (Ser17). Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Cdc25A (also know as M-phase inducer phosphatase 1 and dual specificity phosphatase Cdc25A) is a 65 kD MPI phosphatase containing a rhodanese domain. This nuclear protein functions as a dosage-dependent inducer in mitotic control required for the progression of cell cycle. Cdc25A activates Cdc2 by dephosphorylation, dephosphorylates CDK2 in complex with cyclin E. Cdc25A is timulated by cyclins B, downregulated by Chk1 and Cds1/Chk2 phosphorylation. This phosphatase has been shown to interact with Cdc2, cyclin B, Cdk2, Chk1, and Chk2. The Poly6207 antibody recognizes phosphorylated human Cdc25A (Ser17) and has been shown to be useful for Western blotting.
Dosage-dependent inducer of mitotic control required for progression of cell cycle. Activates Cdc2 by dephosphorylation; dephosphorylates CDK2 in complex with cyclin E
Regulation:
Stimulated by cyclin B, downregulated by Chk1 and Cds1/Chk2 phosphorylation
Modification:
Phosphorylation
Interaction:
Cdc2, cyclin B,Cdk2,Chk1,Chk2
Antigen References:
1. Galaktionov K, et al. 1991. Cell. 67:1181. 2. Jinno S, et al. 1994. EMBO J. 13:1549. 3. Ducruet A, et al. 2003. J. Biol. Chem. 278:31838. 4. Sorensen C, et al. 2003. Cancer Cell. 3:247.
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Purified anti-Cdc25A-Phosphorylated (Ser17)
Hela cells were treated with 300 µM mimosine for 16 hrs, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole (lane 2) for an additional 18hrs. Nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit polyclonal antibody reactive against phosphorylated Cdc25A (Ser17). Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
