This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
MCF-7 cell extract (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal antibody against CBP. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Description:
CBP (also known as CREB-binding protein and CBP/p300) is a widely expressed nuclear protein that contains a bromodomain, a ZZ-type zinc domain, and TAZ-type zinc fingers. This 265 kD acetyltransferase enzyme, acetylates histones and non-histone proteins like NCOA3 coactivator and mediates c-AMP gene regulating by binding to phosphorylated CREB. CBP and phosphorylated CREB activates transcription of c-AMP responsive genes; defects in CBP result in Rubinstein-Taybi syndrome (mental retardation, craniofacial defects, heart defects). CBP is activated by TCR signaling and is involved in TNF-α gene expression and plays a central role in regulating gene responses to hypoxia. This protein is acetylated at multiple sites. CBP has been shown to interact with NCOA6, androgen receptor, STAT6, SRC1, Csk, FKHR, and c-jun and to form a complex with NCOA2, NCOA3, IKKA, IKKB, and IKBKG. In addition, CBP probably forms a complex with HIF1A and EP300. The Poly6064 antibody has been shown to be useful for Western blotting of the human and mouse CBP protein.
Acetyltransferase enzyme, acetylates histones and non-histone proteins like NCOA3 coactivator. Mediates c-AMP gene regulating by binding to phosphorylated CREB. CBP and phosphorylated CREB activates transcription of c-AMP responsive genes; defects result
Regulation:
Activated by TCR signaling and involved in TNF-α gene expression, central role in regulating gene responses to hypoxia
Modification:
Acetylation at multiple sites
Interaction:
SMAD1, SMAD2, SMAD3, PCAF, NCOA6, androgen receptor, STAT6, SRC1, Csk, FKHR, and c-jun, forms a complex with NCOA2, NCOA3, IKKA, IKKB, and IKBKG. Probably forms a complex with HIF1A and EP300
Antigen References:
1. Arany, Z., et al., 1996. PNAS 93:12969. 2. MuRata, T., et al., 2001. Hum. Mol. Genet. 10:1071. 3. Falvo, J., et al., 2000. PNAS 97:3925.
