Human, recognizes 46 kD pro-caspase-9 and 34 kD cleaved caspase-9
Immunogen:
N-terminal fragment of human caspase-9
Formulation:
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation:
The antibody was purified by protein G affinity chromatography
Concentration:
0.5 mg/ml
Storage & Handling:
Upon receipt, store at 4° C.
Application:
WB-Quality Tested IP* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 μg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
A431 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-caspase 9 antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.
Description:
Caspase 9 (also known as ICE-like apoptotic protease 6 (ICE-LAP6), apoptotic protease Mch-6, and apoptotic protease activating factor 3 (Apaf-3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. Pro-Caspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, Bcl-XL, c-IAP1, c-IAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf-1/cytochrome c complex, and following cleavage by Apaf-1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 interacts with BIRC7 and has been shown to cleave PARP and vimentin. The 96-2-22 monoclonal antibody has been shown to be useful for Western blotting of human caspase 9 (46 kD pro-caspase 9 as well as the 34 kD cleaved caspase 9).
Peptidase family C14, CARD domain. Heterotetramer, two isoforms, pro-Casp9 47 kD, active cleaved from Casp9 37 kD
Distribution:
Cytoplasm, when activated mitochondrial
Function:
Involved in caspase activation cascade responsible for apoptosis execution; cleaves/activates Casp3, Casp6
Regulation:
Inhibited by dominant negative isoform, Bcl-XL, c-IAP1, c-IAP2, XIAP, Livin. Activated when recruited to Apaf-1/cytochrome c complex, cleaved by Apaf-1, granzyme B, Casp3, possibly Casp8 and -10 into large p37 and small p10 subunits
Interaction:
BIRC7; cleaves PARP, vimentin
Antigen References:
1. Srinivasula, S., et al., 1996. J. Biol. Chem. 271:27099. 2. Hu, Y., et al., 1998. PNAS 95:4386. 3. Sitailo, L., et al., 2002. J. Biol. Chem. 277:19346. 4. Potokar, M., et al., 2003. FEBS Lett. 544:153.
