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Each lot of this antibody is quality control tested by Western blotting. Western blotting suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Western blot analysis of extracts from Hela cells, using anti-Caspase-9, Poly6342.
Caspase-9 is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. Pro-Caspase-9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase-9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase-3 and Caspase-6. Caspase-9 is inhibited by the dominant negative isoform, Bcl-XL, c-IAP1, c-IAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf-1/cytochrome c complex, and following cleavage by Apaf-1, granzyme B, Caspase-3, possibly Caspase-8 and Caspase-10 into large p37 and small p10 subunits. Caspase-9 interacts with BIRC7 and has been shown to cleave PARP and vimentin. The Poly6342 antibody has been shown to be useful for western blotting ofhuman Human, Mouse and rat caspase-9 (p10) and caspase-9 precursor.
Peptidase family C14, CARD domain. Heterotetramer, two isoforms, pro-Casp9 47 kD, active cleaved from Casp9 37 kD
Distribution:
Cytoplasm, when activated mitochondrial
Regulation:
Inhibited by dominant negative isoform, Bcl-XL, c-IAP1, c-IAP2, XIAP, Livin. Activated when recruited to Apaf-1/cytochrome c complex, cleaved by Apaf-1, granzyme B, Casp3, possibly Casp8 and -10 into large p37 and small p10 subunits
Interaction:
BIRC7; cleaves PARP, vimentin
Bioactivity/Activities:
Involved in caspase activation cascade responsible for apoptosis execution; cleaves/activates Casp3, Casp6
Antigen References:
1. Srinivasula S, et al. 1996. J. Biol. Chem. 271:27099. 2. Hu Y, et al. 1998. P. Natl. Acad. Sci. USA 95:4386. 3. Sitailo L, et al. 2002. J. Biol. Chem. 277:19346. 4. Potokar M, et al. 2003. FEBS Lett. 544:153.
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Purified anti-Caspase-9
Western blot analysis of extracts from Hela cells, using anti-Caspase-9, Poly6342.
