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Purified anti-Caspase-8 Antibody
Purified anti-Caspase-8 Antibody
645501 25 µg ¥21,000     
Product Details
Clone: 1H10
Isotype: Rat IgG2a
Reactivity: Human
Immunogen: Full length caspase-8 fusion protein
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store at 4° C.
Application:

WB - Quality tested
IHC, ELISA - Reported in the literature
Recommended Usage:

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5-1 µg antibody per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
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Application
References:

1. Sanchez I et al. 1999. Neuron. 22:623
2. Li H, et al. 1998. Cell 94:491
Jurkat cell extract was resolved
Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-Caspase-8 (clone 1H10). Proteins were visualized using a goat anti-rat secondary conjugated to HRP and a chemiluminescent detection system.


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Description:

Caspase-8 is involved in the apoptosis mediated by CD95 and TNFR-1. It is suggested that following the triggering of apoptosis by CD95 or TNFR-1, the adaptor molecule FADD interact physically with cytosolic caspase-8 and initiate the apoptotic cascade. The active caspase-8 consists of two subunits of p18 and p10. This antibody should recognize the whole length of human caspase-8 and some isofrms of lower MW.
Other Names: MACH, FLICE, Mch5
Structure: Full length protein of 55kd. Contains two homologous domains of DED (death effector domain) of FADD. The remainder of the protein is similar to the CED-3 subfamily of caspases.
Distribution: Expressed in multiple isoforms in multiple tissues. Relatively high level in peripheral leucocytes.
Function: Caspase-8 is involved in apoptosis mediated by CD95 and TNFR-1.
Antigen
References:

1. Muzio M et al. 1996. Cell 817-827
2. Cohen GM. 1997. Biochem J 326:1
GeneID: 841
Latest Publications: View the latest Caspase-8 articles on HighwirePress.com
UniProt: View information about Caspase-8 on UniProt.org
Keywords: Purified anti-Caspase-8, 1H10, Purified, MACH, FLICE, Mch5 , Human, Immunology, Antibodies
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  • Purified anti-Caspase-8
    Jurkat cell extract was resolved

    Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-Caspase-8 (clone 1H10). Proteins were visualized using a goat anti-rat secondary conjugated to HRP and a chemiluminescent detection system.

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