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Purified anti-Caspase-1 Antibody
Cat. # Size Price  

645101 25 µg $105.00
    
645102 100 µg $225.00
    
Product Details

Clone: 5B10 (See other available formats)
Isotype: Rat IgG2a
Reactivity: Human
Immunogen: Bacterial fusion protein (p20)
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
IHC - Reported in the literature
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1-2µg antibody per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application
References:

  Publication Library
 Image 1
MCF7(1) and Jurkat (2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with mAb anti-capsase 1 (clone 5B10) antibody. Proteins were visualized using a goat anti-rat secondary antibody conjugated to HRP and a chemiluminescence system.


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Antigen Details

Description: Caspase -1 plays a vital role in cleaving the inactive procytokine pro-IL-1 beta to generate the active 17 kd mature form of IL-1beta, a key mediator of inflammation. Active caspase-1 (ICE) is derived a single proenzyme of 45 Kd. An active ICE comprises two subunits of P20 and two subunits of P10 after removal a large amino-terminal pro-domain and a small linker peptide. Caspase-1 is reported to be involved in CD95- mediated apoptosis. Four alternatively spliced variants of caspase-1 have been reported, with mw ranging from 30-48 kd.
Other Names: ICE
Structure: The whole length of Caspase-1 is a 404 amino acid polypeptide chain with MW of 45kd. The active Caspase -1 is a tetramer compariseing two subunits of 20Kd and 10Kd.
Distribution: Predominantly in the cytoplasm, some localized to the external cell membrane.
Function: Caspase-1 is a mediator of the inflammatory response by cleaving the inactive pro-IL1 beta at Asp116/Ala 117 to generate the active 17k mature form of IL-1 beta.
Ligand Receptor: Pro-IL1 beta
Antigen
References:
1. Wilson KP et al. 1994. Nature 370:251
2. Cohen GM:1997 Biochem J 326:1
GeneID: 834
UniProt: View information about Caspase-1 on UniProt.org
Keywords: Purified anti-Caspase-1, 5B10, Purified, ICE, Human, Western Blotting, Immunohistochemistry, Immunology, Antibodies
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Related Protocols

Western Blotting Protocol

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-Caspase-1
    MCF7(1) and Jurkat (2) cell

    MCF7(1) and Jurkat (2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with mAb anti-capsase 1 (clone 5B10) antibody. Proteins were visualized using a goat anti-rat secondary antibody conjugated to HRP and a chemiluminescence system.

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