This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB1
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Kumar, P., et al., 2005. J. Biol. Chem 280:25928.PubMed
HepG2 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-c-Src antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Description:
c-Src (proto-oncogene tyrosine-protein kinase Src) is a 60 kD tyrosine protein kinase family containing both SH2 and SH3 domains. c-Src is expressed at high levels in differentiated cells including neurons, platelets, macrophages, many tumors. c-Src is a lipid-modified signaling molecule (myristolyated) involved in cell-cell interactions, cell migration, and proliferation through the phosphorylation of many substrates including β-catenin, paxillin, and vinculin. Phosphorylation of c-Src on Tyr-526 by Csk (c-Src kinase) represses activity; c-Src is activated by phospholipase D, insulin-like growth factor receptor, EGF-receptor, fibroblast growth factor receptor, and prolactin receptor binding. c-Src has been shown to be phosphorylated on multiple sites and to interact with polyoma virus middle T antigen, PYK2, HSP72, and caveolin. The Poly6054 antibody recognizes the N-terminal region of human and mouse c-Src and has been shown to be useful for Western blotting.
