This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store at -20°C.
Application:
WB - Quality Tested
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Description:
The c-fos protein, also known as FBJ murine viral (v-fos) oncogene homolog , is a 62 kD cellular oncogene. The c-fos protein contains a bZIP region with a DNA-binding domain and a leucine zipper domain. Together with c-jun, c-jun proteins form the heterodimeric AP-1 complex that binds to the TRE elements of cellular and viral genes including collagenase, interleukin 2, stromelysin, SV40 and polyoma among others. c-fos is expressed in a variety of normal adult tissues and has been implicated as a critical transcription factor for skeletal formation and maintenance. This transcription factor is modified by phosphorylation by a number of upstream kinases and has been shown to interact with a variety of other proteins involved in transcriptional regulation. c-fos has been implicated in the development of fibrous bone dysplasia and osteosarcoma. The c-fos protein has been shown to interact with a variety of other proteins including c-jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1. The Poly6244 antibody recognizes human, rat, and mouse c-fos and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry.
c-fos heterodimerizes with c-jun to form the transcription factor AP-1; contains a bZIP region with a DNA-binding domain and a leucine zipper domain; approximately 62 kD.
Distribution:
Expressed in the nucleus of many tissues/cells including placenta, brain, thyroid, central nervous system, lung, endometrium, and B lymphocytes.
Function:
FOS and JUN oncoproteins form dimeric complexes that stimulate transcription of genes containing AP-1 regulatory elements. FOS was the first transcription factor identified to have a critical function in the development of cells required for skeletal form
Regulation:
c-fos levels are highly expressed in human term fetal tissues as compared to normal human tissues. c-fos levels have been reported to be increased in patients with fibrous bone dysplasia.
Modification:
Phosphorylation on multiple sites (threonine, serine) by MAP kinases, ERK2, EphB2, PKA, Rsk.
Interaction:
Interacts with a variety of proteins including c-jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1
Antigen References:
1. Dony, C., et al. 1987. Nature 328:711. 2. Grigoriadis, A. E., et al. 1994. Science 266:443. 3. Glover, J. N. M., et al. 1995. Nature 373:257.
