This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation:
The antibody was purified by protein G affinity chromatography
Storage & Handling:
Upon receipt, store at 4° C.
Application:
WB
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 μg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
The A16 monoclonal antibody recognizes both the middle and long forms of C/EBP β but not the short form.
Application References:
1. Lee, Y.-M., et al.1996. Mol. Cell. Biol. 16:4257 2. Su, et al. 2003. J. Biol. Chem. 278:51150.
Nuclear extracts from P388D1 (IL-1) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-C/EBP ß (clone A16). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system. As shown, this antibody specifically recognizes the LAP isoform of C/EBP ß.
Description:
C/EBP β (also known as CCAAT/enhancer-binding protein beta, interleukin 6-dependent DNA-binding protein, and nuclear factor of interleukin 6 [NF-IL6]) is a member of the basic leucine zipper C/EBP transcription factor family. C/EBP has three isoforms that include C/EBPβ, LAP, and LIP (35 kD, 32 kD, and 20 kD, respectively). This protein is localized in the nucleus and functions as a transcription factor in immune, inflammatory, acute-phase, cytokine, collagen type I gene expression. It is upregulated by growth hormones and has been shown to interact with CEBP-α, CEBP-δ, CEBP-γ, CEBP-ε, and CEBP-?. The C/EBP β protein is known to be modified by phosphorylation and ubiquitination. The A16 monoclonal antibody has been shown to be useful for Western blotting of the mouse 35 kD and 32 kD LAP C/EBP β isoform.
Other Names:
CCAAT/enhancer-binding protein beta, Alpha-1-acid glycoprotein/enhancer binding protein, Interleukin 6-dependent DNA-binding protein (IL-6DBP), Nuclear factor of interleukin 6 (NF-IL6), Liver-enriched transcriptional activator protein (LAP)
