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Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Magaud J-P, et al. 1989. J. Histochem. Cytochem. 37:1517. 2. Ejassi-Lassallette A, et al. 2011. 22:245. PubMed 3. Ma L, et al. 2012 PNAS. 109:2090. PubMed.
Brdu-incorporated Hut-78 cells stained with purified BU20a, followed by anti-mouse IgG FITC
BrdU is a uridine derivative and a structural analog of thymidine that can be incorporated into DNA during the S-phase of the cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval.
Other Names:
Bromodeoxyuridine
Structure:
Uridine derivative that can be incorporated into DNA and substitute for thymidine residues.
Distribution:
Cells can be pulsed labeled with BrdU which will be incorporated into DNA during the synthesis phase of the cell cycle.
Function:
Antibody against BrdU can be used to identify cells undergoing DNA replication during the period of BrdU incorporation.
Antigen References:
1. Gratzner HG. 1982. Science 218:474. 2. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573. 3. Dolbeare F, et al. 1985. Cytometry 6:521.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-BrdU
Human T lymphoblastic leukemia cell line, Hut-78, was pulsed with BrdU for 1 hour, fixed and permeabilized with 70% cold ethanol, and then stained with anti-BrdU (clone Bu20a) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram) by following BioLegend BrdU staining protocol.
Purified anti-BrdU
Brdu-incorporated Hut-78 cells stained with purified BU20a, followed by anti-mouse IgG FITC
Biotin anti-BrdU
Brdu-incorporated Hut-78 cells stained with biotinylated BU20a, followed by Sav-PE
PE anti-BrdU
Brdu-incorporated Hut-78 cells stained with BU20a PE
