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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Garcia JF, et al. 2006. Haematogica 91:467.
A20 (lane 1) and PBMC (lane 2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with anti- BLIMP - 1 antibody, ROS195G. Proteins were visualized using a HRP goat anti-mouse antibody and a chemiluminescence detection system
Blimp-1 is a 98 Kd protein containing five Kuppel-type zinc finger domains that confer sequence specific DNA binding. It has been postulated to be a master regulator of terminal B cell differentiation. In the BCL-1 lymphoma model of differentiation from mature B cells to plasma cells, ectopic expression of Blimp-1 is sufficient to cause terminal differentiation manifested by loss of surface immunoglobulin, IgM secretion, expression of syndecan-1 and cessation of cell division. Recently, it is reported that Blimp-1 is also involved in T cell differentiation and T cell homeostasis and suppression of IL-2 production.
Other Names:
B lymphocyte-induced maturation protein-1, PRDM-1, BLIMP1
Structure:
98 Kd protein containing five Kruppel-type zinc finger domains
Distribution:
Expressed in CD4+ and CD8+ effector/memory T cells and in Foxp3+ T reg cells. In the B cell lineage, it is specifically expressed in the antibody secreting cells.
Function:
A transcription repressor expressed in B and T cells.
Antigen References:
1. Zhao WL, et al. 2008. Blood 111:3867. 2. Climmino L, et al. 2008. J. Immunol. 181:2338. 3. Martins G and Calame K. 2008. Annu Rev Immunol. 26:133.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Blimp-1
A20 (lane 1) and PBMC (lane 2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with anti- BLIMP - 1 antibody, ROS195G. Proteins were visualized using a HRP goat anti-mouse antibody and a chemiluminescence detection system
