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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1.0 µg antibody per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Li H, et al. 1998. Cell 94:491. (WB ELISA)
Jurkat cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-BID antibody (clone 1H11). Proteins were visualized using a goat anti-rat IgG secondary conjugated to HRP and chemiluminescence detection.
BID, a BH3 interacting domain death agonist is a member of the Bcl-2/Bcl-xL family. BID can form a heterodimer with either agonist BAX or antagonist Bcl-2. It is a mediator of mitochondrial damage induced by Caspase-8 (CASP8). After the cleavage by CASP8, the COOH-terminal part translocates to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
Other Names:
BH3 domain-only protein, BH3 interacting domain death agonist
Structure:
195 aa encodes a 22 kD protein. It contains only BH3 domain, not the BH1, BH2, and BH4 domains found in other Bcl-2 family members.
Distribution:
Cytoplasmic, inside mitochondria once activated.
Function:
Activated by Caspase 8 in response to Fas/TNF-R1 death receptor signals.
Ligand Receptor:
Caspase-8, BAK
Antigen References:
1. Wang K, et al. 1996. Genes Dev. 10:2859. 2. Luo X, et al. 1998. Cell 94:481. 3. Yin XM, et al. 1999. Nature 400:886 . 4. Strasser A, et al. 2009. Immunity 30:180.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-BID
Jurkat cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-BID antibody (clone 1H11). Proteins were visualized using a goat anti-rat IgG secondary conjugated to HRP and chemiluminescence detection.
